Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B
المؤلفون المشاركون
Saiful Yazan, Latifah
Ong, Yong Sze
Alexander, Henna Roshini
Syed Alwi, Sharifah Sakinah
Zakarial Ansar, Fatin Hanani
Haron, Aminah Suhaila
Abd Razak, Rohaina
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-16
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Thymoquinone (TQ), a bioactive compound found in Nigella sativa, cannot be orally consumed due to its lipophilicity.
In order to overcome this low bioavailability, TQ is loaded into a colloidal drug carrier known as a nanostructured lipid carrier (NLC).
This study aims to determine the antiproliferative effects of TQ and TQ-NLC on liver cancer cells integrated with the hepatitis B genome, Hep3B.
The Hep3B was treated with TQ or TQ-NLC for 24, 48, and 72 hours via MTT assay.
The results confirm that TQ or TQ-NLC inhibited the growth of Hep3B at IC50 <16.7 μM for 72 hours.
TQ was also found to induce cell cycle arrest at the G1 checkpoint while TQ-NLC induced non-phase-specific cell cycle arrest.
Further analysis using Annexin V staining confirmed the apoptotic induction of TQ or TQ-NLC via activation of caspases-3/7.
In ROS management, TQ acted as a prooxidant (increased the level of ROS), while TQ-NLC acted as an antioxidant (reduced the level of ROS).
Molecular analysis demonstrated that the GSH system and the Nrf2/Keap1 signaling pathway in Hep3B influenced the differential responses of the cells towards TQ or TQ-NLC.
Hence, this study demonstrated that TQ and TQ-NLC have in vitro anticancer effects on the Hep3B.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Haron, Aminah Suhaila& Syed Alwi, Sharifah Sakinah& Saiful Yazan, Latifah& Abd Razak, Rohaina& Ong, Yong Sze& Zakarial Ansar, Fatin Hanani…[et al.]. 2018. Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154422
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Haron, Aminah Suhaila…[et al.]. Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1154422
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Haron, Aminah Suhaila& Syed Alwi, Sharifah Sakinah& Saiful Yazan, Latifah& Abd Razak, Rohaina& Ong, Yong Sze& Zakarial Ansar, Fatin Hanani…[et al.]. Cytotoxic Effect of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) on Liver Cancer Cell Integrated with Hepatitis B Genome, Hep3B. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154422
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154422
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر