Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs
المؤلفون المشاركون
Zhang, Guo-Xing
Yu, Xiao
Zhao, Xiao-Dong
Bao, Rong-Qi
Yu, Jia-Yu
Chen, Jing-Wei
Wu, Ying-Ying
Chen, Chao
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Sera from the rats with Tiaozhi granule treatment were collected.
Human umbilical vein endothelial cells (HUVECs) were incubated with different dosage of sera with Tiaozhi granule for 48 hours.
Rapamycin or angiotensin II was applied to activate autophagy in HUVECs with or without different dosages of sera of Tiaozhi granule.
The mRNA expressions of Atg5, Atg7, Beclin-1, and mammal target of rapamycin (mTOR) were detected by real-time PCR.
Autophagic flux markers (protein expression of LC3, Beclin-1, and p62) were examined by western blot analyses.
The number of autophagosomes was visualized by immunofluorescence analysis with LC3-II labelling.
Results showed that Tiaozhi granule sera increase cell autophagic levels by increase of mRNA of Atg5, Atg7, Beclin-1, and mTOR and increase of autophagic flux and also number of autophagosomes.
However, in response to rapamycin or Ang II stimulation, activated autophagic levels were alleviated by Tiaozhi granule sera by reduction of mRNA of Atg5, Atg7, Beclin-1, mTOR, autophagic flux, and also number of autophagosomes.
Our present data demonstrate that Tiaozhi granule plays a dual role in response to different cell conditions, which is to increase cell autophagy under physiological condition and to suppress cell excessive autophagy under pathological condition.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Ying-Ying& Chen, Chao& Yu, Xiao& Zhao, Xiao-Dong& Bao, Rong-Qi& Yu, Jia-Yu…[et al.]. 2018. Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154477
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Ying-Ying…[et al.]. Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1154477
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Ying-Ying& Chen, Chao& Yu, Xiao& Zhao, Xiao-Dong& Bao, Rong-Qi& Yu, Jia-Yu…[et al.]. Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154477
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154477
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر