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Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs
Joint Authors
Zhang, Guo-Xing
Yu, Xiao
Zhao, Xiao-Dong
Bao, Rong-Qi
Yu, Jia-Yu
Chen, Jing-Wei
Wu, Ying-Ying
Chen, Chao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sera from the rats with Tiaozhi granule treatment were collected.
Human umbilical vein endothelial cells (HUVECs) were incubated with different dosage of sera with Tiaozhi granule for 48 hours.
Rapamycin or angiotensin II was applied to activate autophagy in HUVECs with or without different dosages of sera of Tiaozhi granule.
The mRNA expressions of Atg5, Atg7, Beclin-1, and mammal target of rapamycin (mTOR) were detected by real-time PCR.
Autophagic flux markers (protein expression of LC3, Beclin-1, and p62) were examined by western blot analyses.
The number of autophagosomes was visualized by immunofluorescence analysis with LC3-II labelling.
Results showed that Tiaozhi granule sera increase cell autophagic levels by increase of mRNA of Atg5, Atg7, Beclin-1, and mTOR and increase of autophagic flux and also number of autophagosomes.
However, in response to rapamycin or Ang II stimulation, activated autophagic levels were alleviated by Tiaozhi granule sera by reduction of mRNA of Atg5, Atg7, Beclin-1, mTOR, autophagic flux, and also number of autophagosomes.
Our present data demonstrate that Tiaozhi granule plays a dual role in response to different cell conditions, which is to increase cell autophagy under physiological condition and to suppress cell excessive autophagy under pathological condition.
American Psychological Association (APA)
Wu, Ying-Ying& Chen, Chao& Yu, Xiao& Zhao, Xiao-Dong& Bao, Rong-Qi& Yu, Jia-Yu…[et al.]. 2018. Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154477
Modern Language Association (MLA)
Wu, Ying-Ying…[et al.]. Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1154477
American Medical Association (AMA)
Wu, Ying-Ying& Chen, Chao& Yu, Xiao& Zhao, Xiao-Dong& Bao, Rong-Qi& Yu, Jia-Yu…[et al.]. Effects of Tiaozhi Granule on Regulation of Autophagy Levels in HUVECs. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154477
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154477