Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations
المؤلفون المشاركون
Liu, Huiying
Zhang, Hailan
Li, Meng
Tian, Hongzhong
Tang, Xiaobing
Bai, Yuzuo
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-11
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The study objective was to investigate the role of fibroblast growth factor 9 (Fgf9) in normal and anorectal malformation (ARM) embryos during the development of the anorectum.
Fgf9 expression was assayed in both normal rat embryos and embryos with ARM induced by exposure to ethylenethiourea (ETU) on embryonic day 10 (E10).
Fgf9 expression was assayed by immunohistochemical staining, Western blotting, and real-time quantitative polymerase chain reaction (qRT-PCR).
Immunohistochemical staining revealed spatiotemporal changes in Fgf9 expression between E13 and E16.
Fgf9-positive cells predominated in the mesenchyme of the cloaca on E13 and E14 and at the fusion site of the urorectal septum and cloacal membrane, rectal epithelium, and anal membrane on E15.
Fgf9-positive cells were obviously decreased after the anal membrane ruptured on E16.
Fgf9-positive staining was significantly decreased in embryos with ARM compared with normal embryos from E13 to E15.
The results of Western blots and qRT-PCR were consistent, with significantly increased Fgf9 expression in the hindgut and rectum of normal embryos than in embryos with ARM from E13 to E15.
However, there was no difference between the two groups on E16.
These results suggested that the anorectal embryogenesis might depend on the induction of Fgf9 signal.
The expression of Fgf9 was downregulated in ETU-induced ARM embryos, which might be related to the development of ARM.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Huiying& Zhang, Hailan& Li, Meng& Tian, Hongzhong& Tang, Xiaobing& Bai, Yuzuo. 2019. Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations. Gastroenterology Research and Practice،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1154849
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Huiying…[et al.]. Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations. Gastroenterology Research and Practice No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1154849
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Huiying& Zhang, Hailan& Li, Meng& Tian, Hongzhong& Tang, Xiaobing& Bai, Yuzuo. Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations. Gastroenterology Research and Practice. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1154849
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154849
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر