Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations
Joint Authors
Liu, Huiying
Zhang, Hailan
Li, Meng
Tian, Hongzhong
Tang, Xiaobing
Bai, Yuzuo
Source
Gastroenterology Research and Practice
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The study objective was to investigate the role of fibroblast growth factor 9 (Fgf9) in normal and anorectal malformation (ARM) embryos during the development of the anorectum.
Fgf9 expression was assayed in both normal rat embryos and embryos with ARM induced by exposure to ethylenethiourea (ETU) on embryonic day 10 (E10).
Fgf9 expression was assayed by immunohistochemical staining, Western blotting, and real-time quantitative polymerase chain reaction (qRT-PCR).
Immunohistochemical staining revealed spatiotemporal changes in Fgf9 expression between E13 and E16.
Fgf9-positive cells predominated in the mesenchyme of the cloaca on E13 and E14 and at the fusion site of the urorectal septum and cloacal membrane, rectal epithelium, and anal membrane on E15.
Fgf9-positive cells were obviously decreased after the anal membrane ruptured on E16.
Fgf9-positive staining was significantly decreased in embryos with ARM compared with normal embryos from E13 to E15.
The results of Western blots and qRT-PCR were consistent, with significantly increased Fgf9 expression in the hindgut and rectum of normal embryos than in embryos with ARM from E13 to E15.
However, there was no difference between the two groups on E16.
These results suggested that the anorectal embryogenesis might depend on the induction of Fgf9 signal.
The expression of Fgf9 was downregulated in ETU-induced ARM embryos, which might be related to the development of ARM.
American Psychological Association (APA)
Liu, Huiying& Zhang, Hailan& Li, Meng& Tian, Hongzhong& Tang, Xiaobing& Bai, Yuzuo. 2019. Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations. Gastroenterology Research and Practice،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1154849
Modern Language Association (MLA)
Liu, Huiying…[et al.]. Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations. Gastroenterology Research and Practice No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1154849
American Medical Association (AMA)
Liu, Huiying& Zhang, Hailan& Li, Meng& Tian, Hongzhong& Tang, Xiaobing& Bai, Yuzuo. Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations. Gastroenterology Research and Practice. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1154849
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154849