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Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1)
المؤلفون المشاركون
Othman, Fauziah
Abdullah, Rasedee
Abdul Kadir, Arifah
Nordin, Muhammad Luqman
Zakaria, Zainul Amiruddin
Abdullah, Muhammad Nazrul Hakim
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-16
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
This study was conducted to investigate the cytotoxicity and apoptosis effect of A.
crispa extract and its solvent partition (ethyl acetate and aqueous extract) against Mus musculus mammary carcinoma cell line (4T1).
The normal mouse fibroblast cell line (NIH3T3) was used as comparison for selective cytotoxicity properties.
The cytotoxicity evaluation was assessed using MTT assay.
AO/PI dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis.
Results showed that 80% methanol extract from leaves showed most promising antimammary cancer agent with IC50 value of 42.26±1.82 μg/mL and selective index (SI) value of 10.22.
Ethyl acetate was cytotoxic for both cancer and normal cell while aqueous extract exhibited poor cytotoxic effect.
4T1 cells labelled with AO/PI and Annexin V-FITC and treated with 80% methanol extract demonstrated that the extract induces apoptosis to 4T1 mammary cancer cells.
In conclusion, 80% methanol extract of A.
crispa was selectively cytotoxic towards 4T1 cells but less cytotoxic towards NIH3T3 cells and induced the cancerous cells into apoptotic stage as early as 6 hours.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nordin, Muhammad Luqman& Abdul Kadir, Arifah& Zakaria, Zainul Amiruddin& Othman, Fauziah& Abdullah, Rasedee& Abdullah, Muhammad Nazrul Hakim. 2017. Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1). Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154974
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nordin, Muhammad Luqman…[et al.]. Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1). Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154974
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nordin, Muhammad Luqman& Abdul Kadir, Arifah& Zakaria, Zainul Amiruddin& Othman, Fauziah& Abdullah, Rasedee& Abdullah, Muhammad Nazrul Hakim. Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1). Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154974
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154974
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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