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Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1)
Joint Authors
Othman, Fauziah
Abdullah, Rasedee
Abdul Kadir, Arifah
Nordin, Muhammad Luqman
Zakaria, Zainul Amiruddin
Abdullah, Muhammad Nazrul Hakim
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study was conducted to investigate the cytotoxicity and apoptosis effect of A.
crispa extract and its solvent partition (ethyl acetate and aqueous extract) against Mus musculus mammary carcinoma cell line (4T1).
The normal mouse fibroblast cell line (NIH3T3) was used as comparison for selective cytotoxicity properties.
The cytotoxicity evaluation was assessed using MTT assay.
AO/PI dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis.
Results showed that 80% methanol extract from leaves showed most promising antimammary cancer agent with IC50 value of 42.26±1.82 μg/mL and selective index (SI) value of 10.22.
Ethyl acetate was cytotoxic for both cancer and normal cell while aqueous extract exhibited poor cytotoxic effect.
4T1 cells labelled with AO/PI and Annexin V-FITC and treated with 80% methanol extract demonstrated that the extract induces apoptosis to 4T1 mammary cancer cells.
In conclusion, 80% methanol extract of A.
crispa was selectively cytotoxic towards 4T1 cells but less cytotoxic towards NIH3T3 cells and induced the cancerous cells into apoptotic stage as early as 6 hours.
American Psychological Association (APA)
Nordin, Muhammad Luqman& Abdul Kadir, Arifah& Zakaria, Zainul Amiruddin& Othman, Fauziah& Abdullah, Rasedee& Abdullah, Muhammad Nazrul Hakim. 2017. Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1). Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154974
Modern Language Association (MLA)
Nordin, Muhammad Luqman…[et al.]. Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1). Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154974
American Medical Association (AMA)
Nordin, Muhammad Luqman& Abdul Kadir, Arifah& Zakaria, Zainul Amiruddin& Othman, Fauziah& Abdullah, Rasedee& Abdullah, Muhammad Nazrul Hakim. Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1). Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154974
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154974