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Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway
المؤلفون المشاركون
Liu, Jie
He, Lan
Hu, Jing
Li, Kairui
Zhou, Fangliang
Hu, Mei
Luo, Jingjing
Song, Lan
He, Yingchun
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-09
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis.
Methods.
Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dual-fluorescence flow cytometry analysis, Hoechst 33342 staining, and mitochondrial membrane potential detection kit were used to detect cell apoptosis; western blot was used to detect protein expression.
Results.
Different concentrations of isoimperatorin (10 μM, 20 μM, 30 μM, and 40 μM) significantly inhibited the nasopharyngeal carcinoma CNE2 cell proliferation, and 48 h later, the inhibitory effect of the 40 μM treatment was significantly higher than that of 10 μM and 20 μM.
Treatment for 48 h significantly induced nasopharyngeal carcinoma cell apoptosis and resulted in nuclear pyknosis and fragmentation.
At the same timepoint, the expression levels of proliferation-related protein PCNA as well as antiapoptosis proteins XIAP, survivin, and Bcl-2 were decreased by drug treatment, the expression level of proapoptosis protein Bax was increased, and the expression of the key MAPK/ERK1/2 signaling pathway proteins p-c-Raf, p-MEK, and p-ERK1/2 of were decreased.
After activation of the MAPK/ERK1/2 signaling pathway by isoprenaline hydrochloride (ISO), the efficacy of isoimperatorin to downregulate p-c-Raf, p-MEK, and p-ERK1/2 expressions in the MAPK/ERK1/2 signaling pathway and proliferation-related protein PCNA as well as antiapoptosis proteins XIAP, surviving, and Bcl-2 was reduced compared with that of isoimperatorin alone, the effect of upregulating the proapoptotic protein Bax was reduced, and the apoptosis rate was also decreased.
Conclusion.
Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Jie& He, Lan& Hu, Jing& Li, Kairui& Zhou, Fangliang& Hu, Mei…[et al.]. 2020. Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155088
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Jie…[et al.]. Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1155088
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Jie& He, Lan& Hu, Jing& Li, Kairui& Zhou, Fangliang& Hu, Mei…[et al.]. Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155088
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155088
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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