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Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway
Joint Authors
Liu, Jie
He, Lan
Hu, Jing
Li, Kairui
Zhou, Fangliang
Hu, Mei
Luo, Jingjing
Song, Lan
He, Yingchun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis.
Methods.
Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dual-fluorescence flow cytometry analysis, Hoechst 33342 staining, and mitochondrial membrane potential detection kit were used to detect cell apoptosis; western blot was used to detect protein expression.
Results.
Different concentrations of isoimperatorin (10 μM, 20 μM, 30 μM, and 40 μM) significantly inhibited the nasopharyngeal carcinoma CNE2 cell proliferation, and 48 h later, the inhibitory effect of the 40 μM treatment was significantly higher than that of 10 μM and 20 μM.
Treatment for 48 h significantly induced nasopharyngeal carcinoma cell apoptosis and resulted in nuclear pyknosis and fragmentation.
At the same timepoint, the expression levels of proliferation-related protein PCNA as well as antiapoptosis proteins XIAP, survivin, and Bcl-2 were decreased by drug treatment, the expression level of proapoptosis protein Bax was increased, and the expression of the key MAPK/ERK1/2 signaling pathway proteins p-c-Raf, p-MEK, and p-ERK1/2 of were decreased.
After activation of the MAPK/ERK1/2 signaling pathway by isoprenaline hydrochloride (ISO), the efficacy of isoimperatorin to downregulate p-c-Raf, p-MEK, and p-ERK1/2 expressions in the MAPK/ERK1/2 signaling pathway and proliferation-related protein PCNA as well as antiapoptosis proteins XIAP, surviving, and Bcl-2 was reduced compared with that of isoimperatorin alone, the effect of upregulating the proapoptotic protein Bax was reduced, and the apoptosis rate was also decreased.
Conclusion.
Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway.
American Psychological Association (APA)
Liu, Jie& He, Lan& Hu, Jing& Li, Kairui& Zhou, Fangliang& Hu, Mei…[et al.]. 2020. Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155088
Modern Language Association (MLA)
Liu, Jie…[et al.]. Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1155088
American Medical Association (AMA)
Liu, Jie& He, Lan& Hu, Jing& Li, Kairui& Zhou, Fangliang& Hu, Mei…[et al.]. Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPKERK12 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155088