Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells
المؤلفون المشاركون
Wang, Jingli
Zhu, Congyuan
Li, Jianping
Hua, Yuming
Wang, Ke
Sun, Jingqiu
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-29
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
In this study, we aimed to evaluate the suppressive abilities of berberine (BBR) on MCF-7 and MDA-MB-231 cells and confirm its underlying mechanisms on miR-214-3p.
We first built a panel of 18 miRNAs and 9 lncRNAs that were reported to participate in the mechanism of breast cancer.
The RT-qPCR results suggested that BBR illustrated a dosage-dependent pattern in the stimulation to miR-214-3p in both MCF-7 and MDA-MB-231 cells.
Then, we performed gain-and-lose function tests to validate the role of miR-214-3p contributing to the anticancer effects of BBR.
Both BBR and miR-214-3p mimic reduced the cell viability, repressed migration and invasion capacities, increased rates of total apoptotic cells and ratio of Bax/Bcl-2, and increased the percentage of G2/M cells of MCF-7 and MDA-MB-231 cells by colony formation and CKK8 assay, scratch wound healing and gelatin-based 3D conformation assay, transwell invasion assay, and cell cycle analysis, respectively.
However, miR-214-3p inhibitor counteracted all these effects of BBR.
Based on the bioinformatics analysis and dual-luciferase reporter test, we identified binding sites between SCT and miR-214-3p.
We further confirmed that BBR massively and dose-dependently reduced the mRNA expression and protein levels of SCT in both MCF-7 and MDA-231 cells.
We testified that both miR-214-3p mimic and BBR could decrease the mRNA expression and protein levels of SCT, while miR-214-3p inhibitor weakened these reductions.
In conclusion, BBR suppressed MCF-7 and MDA-MB-231 breast cancer cells by upregulating miR-214-3p and increasing its inhibition to SCT.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhu, Congyuan& Li, Jianping& Hua, Yuming& Wang, Jingli& Wang, Ke& Sun, Jingqiu. 2020. Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155433
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhu, Congyuan…[et al.]. Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1155433
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhu, Congyuan& Li, Jianping& Hua, Yuming& Wang, Jingli& Wang, Ke& Sun, Jingqiu. Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155433
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155433
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر