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Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells
Joint Authors
Wang, Jingli
Zhu, Congyuan
Li, Jianping
Hua, Yuming
Wang, Ke
Sun, Jingqiu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In this study, we aimed to evaluate the suppressive abilities of berberine (BBR) on MCF-7 and MDA-MB-231 cells and confirm its underlying mechanisms on miR-214-3p.
We first built a panel of 18 miRNAs and 9 lncRNAs that were reported to participate in the mechanism of breast cancer.
The RT-qPCR results suggested that BBR illustrated a dosage-dependent pattern in the stimulation to miR-214-3p in both MCF-7 and MDA-MB-231 cells.
Then, we performed gain-and-lose function tests to validate the role of miR-214-3p contributing to the anticancer effects of BBR.
Both BBR and miR-214-3p mimic reduced the cell viability, repressed migration and invasion capacities, increased rates of total apoptotic cells and ratio of Bax/Bcl-2, and increased the percentage of G2/M cells of MCF-7 and MDA-MB-231 cells by colony formation and CKK8 assay, scratch wound healing and gelatin-based 3D conformation assay, transwell invasion assay, and cell cycle analysis, respectively.
However, miR-214-3p inhibitor counteracted all these effects of BBR.
Based on the bioinformatics analysis and dual-luciferase reporter test, we identified binding sites between SCT and miR-214-3p.
We further confirmed that BBR massively and dose-dependently reduced the mRNA expression and protein levels of SCT in both MCF-7 and MDA-231 cells.
We testified that both miR-214-3p mimic and BBR could decrease the mRNA expression and protein levels of SCT, while miR-214-3p inhibitor weakened these reductions.
In conclusion, BBR suppressed MCF-7 and MDA-MB-231 breast cancer cells by upregulating miR-214-3p and increasing its inhibition to SCT.
American Psychological Association (APA)
Zhu, Congyuan& Li, Jianping& Hua, Yuming& Wang, Jingli& Wang, Ke& Sun, Jingqiu. 2020. Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155433
Modern Language Association (MLA)
Zhu, Congyuan…[et al.]. Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1155433
American Medical Association (AMA)
Zhu, Congyuan& Li, Jianping& Hua, Yuming& Wang, Jingli& Wang, Ke& Sun, Jingqiu. Berberine Inhibits the Expression of SCT through miR-214-3p Stimulation in Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155433