A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva
المؤلفون المشاركون
Alvi, Syed N.
Hammami, Muhammad M.
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A simple ultraperformance liquid chromatography-tandem mass spectrometry assay for measurement of cortisol level in human saliva was developed and validated.
Saliva samples containing cortisol were spiked with tolperisone as internal standard (IS) and extracted with a mixture of methyl tert-butyl ether and hexane (8:2, v:v).
After solvent evaporation, residue was reconstituted in 100 μl mobile phase.
Analysis was performed on Atlantis dC18 column (2.1 × 100 mm, 3 μm particle size) with a mobile phase composed of acetonitrile and 2 mM ammonium acetate (50:50, v:v) and delivered at a flow rate of 0.3 ml/minute.
Mass spectrometry acquisition was performed with multiple reaction monitoring in positive-ion mode for cortisol and IS (m/z: 363.1 → 121.0 and 246.0 → 97.9, respectively).
Retention times of cortisol and IS were about 1.35 and 2.45 minutes, respectively.
The relationship between cortisol level and peak area ratio of cortisol to IS was linear in the range of 0.5-100 ng/ml.
Intra- and interday coefficient of variation and bias were ≤ 9.0% and ≤12.0%, respectively.
Mean extraction recoveries of cortisol and IS from saliva samples were 92% and 94%, respectively.
Using the method, cortisol was found to be ≥ 86% stable in processed (24 hours at room temperature or 48 hours at -20°C) and ≥ 91% stable in unprocessed (24 hours at room temperature or 20 weeks at -20°C) saliva samples.
Further, the method was successfully applied to determine daily cortisol profile in saliva samples of a healthy volunteer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alvi, Syed N.& Hammami, Muhammad M.. 2019. A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1155899
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alvi, Syed N.& Hammami, Muhammad M.. A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1155899
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alvi, Syed N.& Hammami, Muhammad M.. A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1155899
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155899
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر