![](/images/graphics-bg.png)
ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells
المؤلفون المشاركون
Tan, Bee Ling
Norhaizan, Mohd Esa
Chan, Lee Chin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-06
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Manilkara zapota (L.) P.
Royen (family: Sapotaceae) is commonly called sapodilla, or locally known as ciku.
The detailed mechanisms underlying Manilkara zapota leaf methanol extract against HeLa human cervical cancer cells have yet to be investigated.
Therefore, our present study is designed to investigate the ability to induce apoptosis and the underlying mechanisms of Manilkara zapota leaf methanol extract inducing cytotoxicity in HeLa cells.
The apoptotic cell death was assessed using Annexin V-propidium iodide staining.
Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential activities were measured using dichlorodihydrofluorescein diacetate and MitoLite Orange, respectively, by NovoCyte Flow Cytometer.
Bax and Bcl-2 expression were evaluated using Enzyme-Linked Immunosorbent Assay.
Caspase-3 activity was determined using a colorimetric assay.
The associated biological interaction pathways were evaluated using quantitative real-time PCR.
Our data showed that HeLa cells were relatively more sensitive to Manilkara zapota leaf methanol extract than other cancer cell lines studied.
Overall analyses revealed that Manilkara zapota leaf methanol extract can inhibit the viability of HeLa cells, induce mitochondrial ROS generation, and inhibit nuclear factor-kappa B (NF-κB) and epidermal growth factor receptor (EGFR) transcriptional activities.
Our results suggested that Manilkara zapota leaf methanol extract might represent a potential anticervical cancer agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tan, Bee Ling& Norhaizan, Mohd Esa& Chan, Lee Chin. 2018. ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-19.
https://search.emarefa.net/detail/BIM-1155944
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tan, Bee Ling…[et al.]. ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-19.
https://search.emarefa.net/detail/BIM-1155944
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tan, Bee Ling& Norhaizan, Mohd Esa& Chan, Lee Chin. ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-19.
https://search.emarefa.net/detail/BIM-1155944
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1155944
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)