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ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells
Joint Authors
Tan, Bee Ling
Norhaizan, Mohd Esa
Chan, Lee Chin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-06
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Manilkara zapota (L.) P.
Royen (family: Sapotaceae) is commonly called sapodilla, or locally known as ciku.
The detailed mechanisms underlying Manilkara zapota leaf methanol extract against HeLa human cervical cancer cells have yet to be investigated.
Therefore, our present study is designed to investigate the ability to induce apoptosis and the underlying mechanisms of Manilkara zapota leaf methanol extract inducing cytotoxicity in HeLa cells.
The apoptotic cell death was assessed using Annexin V-propidium iodide staining.
Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential activities were measured using dichlorodihydrofluorescein diacetate and MitoLite Orange, respectively, by NovoCyte Flow Cytometer.
Bax and Bcl-2 expression were evaluated using Enzyme-Linked Immunosorbent Assay.
Caspase-3 activity was determined using a colorimetric assay.
The associated biological interaction pathways were evaluated using quantitative real-time PCR.
Our data showed that HeLa cells were relatively more sensitive to Manilkara zapota leaf methanol extract than other cancer cell lines studied.
Overall analyses revealed that Manilkara zapota leaf methanol extract can inhibit the viability of HeLa cells, induce mitochondrial ROS generation, and inhibit nuclear factor-kappa B (NF-κB) and epidermal growth factor receptor (EGFR) transcriptional activities.
Our results suggested that Manilkara zapota leaf methanol extract might represent a potential anticervical cancer agent.
American Psychological Association (APA)
Tan, Bee Ling& Norhaizan, Mohd Esa& Chan, Lee Chin. 2018. ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-19.
https://search.emarefa.net/detail/BIM-1155944
Modern Language Association (MLA)
Tan, Bee Ling…[et al.]. ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-19.
https://search.emarefa.net/detail/BIM-1155944
American Medical Association (AMA)
Tan, Bee Ling& Norhaizan, Mohd Esa& Chan, Lee Chin. ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFRNF-κB Activities of HeLa Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-19.
https://search.emarefa.net/detail/BIM-1155944
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155944