Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method
المؤلفون المشاركون
El-Alfy, Walaa
Ismaiel, Omnia A.
El-Mammli, Magda Y.
Shalaby, Abadalla
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A simple RP-HPLC-PDA method for determination of atenolol (ATN) and trimetazidine (TMZ) in human urine and tablets has been developed.
Analytes were separated on a Caltrex BI column (125× 4.0 mm, 5 μm) with 25mM potassium dihydrogen phosphate pH 3.3, methanol, and acetonitrile mobile phases.
The PDA detector was operated at 210 nm for TMZ and 225 nm for ATN and the flow rate was 1.0 mL/ min.
Linearity was obtained over a concentration range of (1.0-100 μg/mL) for both analytes in standard solutions and the method was successfully applied for determination of target analytes in their pharmaceutical tablets.
Excellent linearity was also obtained over concentration ranges of (0.25-25 μg/mL) and (0.5-25 μg/mL) in human urine for TMZ and ATN, respectively.
A simple liquid-liquid extraction was applied for urine sample clean-up and a gradient method was used for chromatographic separation.
The lower limit of quantitation (LOQ) was 0.99 and 0.60 μg/mL for ATN and TMZ, respectively.
The limit of detection (LOD) was 0.30 and 0.18 μg/mL for ATN and TMZ, respectively.
Inter- and intraday precision and accuracy for ATN were within ±1.89% in pure form and within ±2.85% in urine samples.
Inter- and intraday precision and accuracy for TMZ were within ± 3.99% in pure form and within ± 3.19% in urine samples.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
El-Alfy, Walaa& Ismaiel, Omnia A.& El-Mammli, Magda Y.& Shalaby, Abadalla. 2019. Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1156012
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
El-Alfy, Walaa…[et al.]. Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1156012
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
El-Alfy, Walaa& Ismaiel, Omnia A.& El-Mammli, Magda Y.& Shalaby, Abadalla. Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1156012
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156012
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر