Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method
Joint Authors
El-Alfy, Walaa
Ismaiel, Omnia A.
El-Mammli, Magda Y.
Shalaby, Abadalla
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A simple RP-HPLC-PDA method for determination of atenolol (ATN) and trimetazidine (TMZ) in human urine and tablets has been developed.
Analytes were separated on a Caltrex BI column (125× 4.0 mm, 5 μm) with 25mM potassium dihydrogen phosphate pH 3.3, methanol, and acetonitrile mobile phases.
The PDA detector was operated at 210 nm for TMZ and 225 nm for ATN and the flow rate was 1.0 mL/ min.
Linearity was obtained over a concentration range of (1.0-100 μg/mL) for both analytes in standard solutions and the method was successfully applied for determination of target analytes in their pharmaceutical tablets.
Excellent linearity was also obtained over concentration ranges of (0.25-25 μg/mL) and (0.5-25 μg/mL) in human urine for TMZ and ATN, respectively.
A simple liquid-liquid extraction was applied for urine sample clean-up and a gradient method was used for chromatographic separation.
The lower limit of quantitation (LOQ) was 0.99 and 0.60 μg/mL for ATN and TMZ, respectively.
The limit of detection (LOD) was 0.30 and 0.18 μg/mL for ATN and TMZ, respectively.
Inter- and intraday precision and accuracy for ATN were within ±1.89% in pure form and within ±2.85% in urine samples.
Inter- and intraday precision and accuracy for TMZ were within ± 3.99% in pure form and within ± 3.19% in urine samples.
American Psychological Association (APA)
El-Alfy, Walaa& Ismaiel, Omnia A.& El-Mammli, Magda Y.& Shalaby, Abadalla. 2019. Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1156012
Modern Language Association (MLA)
El-Alfy, Walaa…[et al.]. Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1156012
American Medical Association (AMA)
El-Alfy, Walaa& Ismaiel, Omnia A.& El-Mammli, Magda Y.& Shalaby, Abadalla. Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1156012
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156012