Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes
المؤلفون المشاركون
Omiya, Yuji
Ueki, Toshiyuki
Mizoguchi, Kazushige
Kawakami, Zenji
Kanno, Hitomi
Yamamoto, Masahiro
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-06
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Astrocytes carry two glutamate transporters—GLAST and GLT-1—the latter of which is responsible for >90% of glutamate uptake activity in the brain; however, under culture conditions, the GLT-1 expression in astrocytes is exceedingly low, as is the glutamate uptake activity mediated by GLT-1.
This study aimed to elucidate the effects of yokukansan (YKS) in relation to the GLT-1-mediated regulation of extracellular glutamate concentrations.
Thus, we treated cultured astrocytes with tumor necrosis factor-α (TNF-α) and dibutyryl-cAMP (dBcAMP) (hereinafter, referred to as “TA”) to increase GLT-1 expression and then functionally examined how YKS would affect glutamate uptake ability derived from GLT-1.
Contrary to expectations, although the TA treatments did not affect the uptake activity, YKS significantly augmented it.
Conversely, GLAST-derived glutamate uptake was significantly reduced by TA treatments but was unaffected by YKS.
Subsequently, we analyzed the GLT-1 protein and mRNA levels and found that TA treatments had significantly increased them, which were then further augmented by YKS.
These findings suggest that YKS enhances GLT-1-derived glutamate transport functions in TA-treated cultured astrocytes and that this process entails increased GLT-1 protein and mRNA levels.
This type of mechanism may contribute to the YKS-mediated regulation of extracellular glutamate concentrations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ueki, Toshiyuki& Kawakami, Zenji& Kanno, Hitomi& Omiya, Yuji& Mizoguchi, Kazushige& Yamamoto, Masahiro. 2018. Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1156030
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ueki, Toshiyuki…[et al.]. Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1156030
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ueki, Toshiyuki& Kawakami, Zenji& Kanno, Hitomi& Omiya, Yuji& Mizoguchi, Kazushige& Yamamoto, Masahiro. Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1156030
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156030
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر