Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes
Joint Authors
Omiya, Yuji
Ueki, Toshiyuki
Mizoguchi, Kazushige
Kawakami, Zenji
Kanno, Hitomi
Yamamoto, Masahiro
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Astrocytes carry two glutamate transporters—GLAST and GLT-1—the latter of which is responsible for >90% of glutamate uptake activity in the brain; however, under culture conditions, the GLT-1 expression in astrocytes is exceedingly low, as is the glutamate uptake activity mediated by GLT-1.
This study aimed to elucidate the effects of yokukansan (YKS) in relation to the GLT-1-mediated regulation of extracellular glutamate concentrations.
Thus, we treated cultured astrocytes with tumor necrosis factor-α (TNF-α) and dibutyryl-cAMP (dBcAMP) (hereinafter, referred to as “TA”) to increase GLT-1 expression and then functionally examined how YKS would affect glutamate uptake ability derived from GLT-1.
Contrary to expectations, although the TA treatments did not affect the uptake activity, YKS significantly augmented it.
Conversely, GLAST-derived glutamate uptake was significantly reduced by TA treatments but was unaffected by YKS.
Subsequently, we analyzed the GLT-1 protein and mRNA levels and found that TA treatments had significantly increased them, which were then further augmented by YKS.
These findings suggest that YKS enhances GLT-1-derived glutamate transport functions in TA-treated cultured astrocytes and that this process entails increased GLT-1 protein and mRNA levels.
This type of mechanism may contribute to the YKS-mediated regulation of extracellular glutamate concentrations.
American Psychological Association (APA)
Ueki, Toshiyuki& Kawakami, Zenji& Kanno, Hitomi& Omiya, Yuji& Mizoguchi, Kazushige& Yamamoto, Masahiro. 2018. Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1156030
Modern Language Association (MLA)
Ueki, Toshiyuki…[et al.]. Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1156030
American Medical Association (AMA)
Ueki, Toshiyuki& Kawakami, Zenji& Kanno, Hitomi& Omiya, Yuji& Mizoguchi, Kazushige& Yamamoto, Masahiro. Yokukansan, a Traditional Japanese Medicine, Enhances the Glutamate Transporter GLT-1 Function in Cultured Rat Cortical Astrocytes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1156030
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156030