Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract
المؤلفون المشاركون
Shakoor, Hira
Abdelfattah, Fatima
Albadi, Khaula
Adib, Mentalla
Kizhakkayil, Jaleel
Platat, Carine
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Type 2 diabetes mellitus is increasing worldwide, and the United Arab Emirates is presenting one of the world’s highest prevalence rates.
Dietary polyphenols exert an antidiabetic effect by modulating carbohydrates digestion and cellular glucose uptake.
Due to their particularly high content in polyphenols, date seeds represent a potential antidiabetic agent.
This study aims to determine if date seed polyphenols inhibit the activity of the enzymes (α-amylase and α-glucosidase), responsible for the digestion of carbohydrates and modulating the glucose uptake by human liver cells.
In vitro activity of the intestinal α-glucosidase, pancreatic α-amylase, the glucose uptake by HepG2 cells, and the expression of GLUT4 and AMPK analyzed by western blotting (with and without date seeds extract).
Our result showed that the maximum enzymes inhibition was obtained with 400 μg/mL and 900 μg/mL DSE for α-amylase and α-glucosidase, respectively.
The HepG2 cell viability significantly decreased up to 80% at 4000 μg/mL DSE.
The expression of GLUT4 was higher at 100 μg/mL DSE (with insulin and without insulin).
However, the expressions of P-AMPK and AMPK were increased by DSE, mainly in a non-insulin-dependent manner.
Therefore, DSE, by inhibiting carbohydrate digestion and stimulating glucose uptake by HepG2, can potentially demonstrate the therapeutic potential for diabetes management.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shakoor, Hira& Abdelfattah, Fatima& Albadi, Khaula& Adib, Mentalla& Kizhakkayil, Jaleel& Platat, Carine. 2020. Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156088
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shakoor, Hira…[et al.]. Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156088
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shakoor, Hira& Abdelfattah, Fatima& Albadi, Khaula& Adib, Mentalla& Kizhakkayil, Jaleel& Platat, Carine. Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156088
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156088
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر