![](/images/graphics-bg.png)
Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract
Joint Authors
Shakoor, Hira
Abdelfattah, Fatima
Albadi, Khaula
Adib, Mentalla
Kizhakkayil, Jaleel
Platat, Carine
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Type 2 diabetes mellitus is increasing worldwide, and the United Arab Emirates is presenting one of the world’s highest prevalence rates.
Dietary polyphenols exert an antidiabetic effect by modulating carbohydrates digestion and cellular glucose uptake.
Due to their particularly high content in polyphenols, date seeds represent a potential antidiabetic agent.
This study aims to determine if date seed polyphenols inhibit the activity of the enzymes (α-amylase and α-glucosidase), responsible for the digestion of carbohydrates and modulating the glucose uptake by human liver cells.
In vitro activity of the intestinal α-glucosidase, pancreatic α-amylase, the glucose uptake by HepG2 cells, and the expression of GLUT4 and AMPK analyzed by western blotting (with and without date seeds extract).
Our result showed that the maximum enzymes inhibition was obtained with 400 μg/mL and 900 μg/mL DSE for α-amylase and α-glucosidase, respectively.
The HepG2 cell viability significantly decreased up to 80% at 4000 μg/mL DSE.
The expression of GLUT4 was higher at 100 μg/mL DSE (with insulin and without insulin).
However, the expressions of P-AMPK and AMPK were increased by DSE, mainly in a non-insulin-dependent manner.
Therefore, DSE, by inhibiting carbohydrate digestion and stimulating glucose uptake by HepG2, can potentially demonstrate the therapeutic potential for diabetes management.
American Psychological Association (APA)
Shakoor, Hira& Abdelfattah, Fatima& Albadi, Khaula& Adib, Mentalla& Kizhakkayil, Jaleel& Platat, Carine. 2020. Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156088
Modern Language Association (MLA)
Shakoor, Hira…[et al.]. Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156088
American Medical Association (AMA)
Shakoor, Hira& Abdelfattah, Fatima& Albadi, Khaula& Adib, Mentalla& Kizhakkayil, Jaleel& Platat, Carine. Inhibition of Digestive Enzyme and Stimulation of Human Liver Cells (HepG2) Glucose Uptake by Date Seeds Extract. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156088