The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas
المؤلفون المشاركون
Zhao, Zhenzhen
Li, Jing
Liu, Wen
Che, Kui
Zhang, Yan
Luo, Bing
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-04-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Epstein-Barr virus (EBV) is an important DNA virus which establishes latent infection in human malignancies.
Expression of EBV-encoded genes in the associated tumors is strongly modulated by promoter CpG methylation of EBV genome.
This study aimed to explore the methylation status of the promoters of EBV BamHI-A rightward frame 1 (BARF1) and BamHI-H rightward open reading frame 1 (BHRF1) and their influence on transcriptional expression, to further understand the roles of BARF1 and BHRF1 in the occurrence of EBV-associated cancer.
We evaluated the methylation status of BARF1 and BHRF1 promoters in 43 EBV-associated gastric carcinoma (EBVaGC) tissues and EBV-positive cell lines.
Their expressions were evaluated by real-time quantitative PCR.
We found that the promoters of BARF1 and BHRF1 were methylated by varying degrees in different EBV-positive cell lines and were almost hypermethylated in all EBVaGC tissues.
The methylation status of BARF1 and BHRF1 promoters were significantly reduced by 5-Aza-CdR along with the increasing gene expressions.
Hypermethylation of Ap and Hp mediates the frequent silencing of BARF1 and BHRF1 in EBV-associated tumors, which could be reactivated by a demethylation agent, suggesting that promoter demethylation and activation is important for BARF1 and BHRF1 transcription and their further action.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Jing& Liu, Wen& Che, Kui& Zhang, Yan& Zhao, Zhenzhen& Luo, Bing. 2017. The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1156344
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Jing…[et al.]. The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas. Gastroenterology Research and Practice No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1156344
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Jing& Liu, Wen& Che, Kui& Zhang, Yan& Zhao, Zhenzhen& Luo, Bing. The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1156344
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156344
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر