The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas
Joint Authors
Zhao, Zhenzhen
Li, Jing
Liu, Wen
Che, Kui
Zhang, Yan
Luo, Bing
Source
Gastroenterology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Epstein-Barr virus (EBV) is an important DNA virus which establishes latent infection in human malignancies.
Expression of EBV-encoded genes in the associated tumors is strongly modulated by promoter CpG methylation of EBV genome.
This study aimed to explore the methylation status of the promoters of EBV BamHI-A rightward frame 1 (BARF1) and BamHI-H rightward open reading frame 1 (BHRF1) and their influence on transcriptional expression, to further understand the roles of BARF1 and BHRF1 in the occurrence of EBV-associated cancer.
We evaluated the methylation status of BARF1 and BHRF1 promoters in 43 EBV-associated gastric carcinoma (EBVaGC) tissues and EBV-positive cell lines.
Their expressions were evaluated by real-time quantitative PCR.
We found that the promoters of BARF1 and BHRF1 were methylated by varying degrees in different EBV-positive cell lines and were almost hypermethylated in all EBVaGC tissues.
The methylation status of BARF1 and BHRF1 promoters were significantly reduced by 5-Aza-CdR along with the increasing gene expressions.
Hypermethylation of Ap and Hp mediates the frequent silencing of BARF1 and BHRF1 in EBV-associated tumors, which could be reactivated by a demethylation agent, suggesting that promoter demethylation and activation is important for BARF1 and BHRF1 transcription and their further action.
American Psychological Association (APA)
Li, Jing& Liu, Wen& Che, Kui& Zhang, Yan& Zhao, Zhenzhen& Luo, Bing. 2017. The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1156344
Modern Language Association (MLA)
Li, Jing…[et al.]. The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas. Gastroenterology Research and Practice No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1156344
American Medical Association (AMA)
Li, Jing& Liu, Wen& Che, Kui& Zhang, Yan& Zhao, Zhenzhen& Luo, Bing. The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1156344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156344