Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2
المؤلفون المشاركون
Ma, Yi
Ma, Min
Yan, Xianxin
Lin, Shujun
Jiang, Xuefeng
Zhang, Guijuan
Wu, Jieyan
Liu, Yusheng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-04
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Purpose.
Aloe-emodin (AE) is a natural compound derived from aloe vera and palmatum rhubarb and shows anticancer activities in various cancers.
Bcl-2 family is the main regulator of cell death or cell survival.
This study describes the effects of AE on proliferation of breast tumor (BT) cells.
Methods.
MCF-10A, MCF-10AT, MCF-7, and MDA-MB-231 cell lines were exposed to AE.
Cell proliferation and apoptosis were assessed by CCK-8 and flow cytometry.
Protein levels were measured by Western blotting.
The levels of mRNA and miRNA were examined by RT-PCR.
Bioinformatics was applied to screen miRNAs that bind to 3′-UTR of mRNA.
Results.
The results showed that AE selective activity inhibited the proliferation and induced apoptosis of MCF-10AT and MCF-7 cells but exhibited no significant inhibition in MCF10A and MDA-MB-231 cells.
Mechanistically, AE dose-dependently decreased the protein expression of Bcl-2 and Bcl-xl, while it increased Bax protein expression in MCF-10AT and MCF-7 cells.
The levels of Bcl-xl and Bax mRNA were altered by AE treatment, which was consistent with the protein expression results.
However, Bcl-2 mRNA levels were not affected in either cell line, suggesting that AE may modulate the protein translation of Bcl-2 through miRNAs.
In all candidate miRNAs that bind to 3′-UTR of Bcl-2, miR-15a and miR-16-1 were dose-dependently downregulated by AE.
Moreover, inhibition of miR-15a/16-1 could eliminate the inhibition of MCF-10AT and MCF-7 cells growth by AE and could reverse the downregulation of AE-induced Bcl-2 protein level.
Conclusion.
Our research provides an important basis that AE induces BT cell apoptosis through upregulation of miR-15a/miR-16-1 that suppresses BCL2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Xuefeng& Liu, Yusheng& Zhang, Guijuan& Lin, Shujun& Wu, Jieyan& Yan, Xianxin…[et al.]. 2020. Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156418
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Xuefeng…[et al.]. Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156418
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Xuefeng& Liu, Yusheng& Zhang, Guijuan& Lin, Shujun& Wu, Jieyan& Yan, Xianxin…[et al.]. Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156418
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156418
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر