Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2
Joint Authors
Ma, Yi
Ma, Min
Yan, Xianxin
Lin, Shujun
Jiang, Xuefeng
Zhang, Guijuan
Wu, Jieyan
Liu, Yusheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
Aloe-emodin (AE) is a natural compound derived from aloe vera and palmatum rhubarb and shows anticancer activities in various cancers.
Bcl-2 family is the main regulator of cell death or cell survival.
This study describes the effects of AE on proliferation of breast tumor (BT) cells.
Methods.
MCF-10A, MCF-10AT, MCF-7, and MDA-MB-231 cell lines were exposed to AE.
Cell proliferation and apoptosis were assessed by CCK-8 and flow cytometry.
Protein levels were measured by Western blotting.
The levels of mRNA and miRNA were examined by RT-PCR.
Bioinformatics was applied to screen miRNAs that bind to 3′-UTR of mRNA.
Results.
The results showed that AE selective activity inhibited the proliferation and induced apoptosis of MCF-10AT and MCF-7 cells but exhibited no significant inhibition in MCF10A and MDA-MB-231 cells.
Mechanistically, AE dose-dependently decreased the protein expression of Bcl-2 and Bcl-xl, while it increased Bax protein expression in MCF-10AT and MCF-7 cells.
The levels of Bcl-xl and Bax mRNA were altered by AE treatment, which was consistent with the protein expression results.
However, Bcl-2 mRNA levels were not affected in either cell line, suggesting that AE may modulate the protein translation of Bcl-2 through miRNAs.
In all candidate miRNAs that bind to 3′-UTR of Bcl-2, miR-15a and miR-16-1 were dose-dependently downregulated by AE.
Moreover, inhibition of miR-15a/16-1 could eliminate the inhibition of MCF-10AT and MCF-7 cells growth by AE and could reverse the downregulation of AE-induced Bcl-2 protein level.
Conclusion.
Our research provides an important basis that AE induces BT cell apoptosis through upregulation of miR-15a/miR-16-1 that suppresses BCL2.
American Psychological Association (APA)
Jiang, Xuefeng& Liu, Yusheng& Zhang, Guijuan& Lin, Shujun& Wu, Jieyan& Yan, Xianxin…[et al.]. 2020. Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156418
Modern Language Association (MLA)
Jiang, Xuefeng…[et al.]. Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156418
American Medical Association (AMA)
Jiang, Xuefeng& Liu, Yusheng& Zhang, Guijuan& Lin, Shujun& Wu, Jieyan& Yan, Xianxin…[et al.]. Aloe-Emodin Induces Breast Tumor Cell Apoptosis through Upregulation of miR-15amiR-16-1 That Suppresses BCL2. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156418