An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia
المؤلفون المشاركون
Zhang, Yuanyuan
Chen, Qiliang
Liu, Yaojun
Jiang, Xinyu
Zhang, Yuqin
Asakawa, Tetsuya
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
The neuroprotective effects of Paeoniflorin (PF) are well known.
Most of the evidence was verified in vivo.
We attempted to perform an in vitro verification of the effects of PF in microglia.
Methods.
A lipopolysaccharide- (LPS-) exposed microglia model was employed.
An enzyme-linked immunosorbent assay was used to measure the levels of cytokines in the culture supernatants.
A real-time polymerase chain reaction was performed to measure the mRNA expression of cytokines and M1- and M2-like genes.
A western blot analysis was used to examine the expression of proteins associated with the nuclear factor-kappa B (NF-κB) signaling pathway.
Results.
We found that the administration of PF reversed the inflammatory response induced by LPS.
It downregulated proinflammatory cytokines and upregulated anti-inflammatory cytokines.
This, in turn, alleviated the oxidative injuries, downregulated the expression of M1-like genes, and upregulated the expression of M2-like genes.
PF can also reverse the changes in proteins associated with the NF-κB signaling pathway induced by LPS.
Conclusions.
We provided evidence obtained in vitro concerning the neuroprotective effects of PF via suppressing activation of microglia, which might be associated with the NF-κB signaling pathway.
These findings contribute to obtaining a deeper understanding of PF, a potential new treatment for brain injuries.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Qiliang& Liu, Yaojun& Zhang, Yuanyuan& Jiang, Xinyu& Zhang, Yuqin& Asakawa, Tetsuya. 2020. An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156713
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Qiliang…[et al.]. An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156713
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Qiliang& Liu, Yaojun& Zhang, Yuanyuan& Jiang, Xinyu& Zhang, Yuqin& Asakawa, Tetsuya. An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156713
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156713
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر