An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia
Joint Authors
Zhang, Yuanyuan
Chen, Qiliang
Liu, Yaojun
Jiang, Xinyu
Zhang, Yuqin
Asakawa, Tetsuya
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
The neuroprotective effects of Paeoniflorin (PF) are well known.
Most of the evidence was verified in vivo.
We attempted to perform an in vitro verification of the effects of PF in microglia.
Methods.
A lipopolysaccharide- (LPS-) exposed microglia model was employed.
An enzyme-linked immunosorbent assay was used to measure the levels of cytokines in the culture supernatants.
A real-time polymerase chain reaction was performed to measure the mRNA expression of cytokines and M1- and M2-like genes.
A western blot analysis was used to examine the expression of proteins associated with the nuclear factor-kappa B (NF-κB) signaling pathway.
Results.
We found that the administration of PF reversed the inflammatory response induced by LPS.
It downregulated proinflammatory cytokines and upregulated anti-inflammatory cytokines.
This, in turn, alleviated the oxidative injuries, downregulated the expression of M1-like genes, and upregulated the expression of M2-like genes.
PF can also reverse the changes in proteins associated with the NF-κB signaling pathway induced by LPS.
Conclusions.
We provided evidence obtained in vitro concerning the neuroprotective effects of PF via suppressing activation of microglia, which might be associated with the NF-κB signaling pathway.
These findings contribute to obtaining a deeper understanding of PF, a potential new treatment for brain injuries.
American Psychological Association (APA)
Chen, Qiliang& Liu, Yaojun& Zhang, Yuanyuan& Jiang, Xinyu& Zhang, Yuqin& Asakawa, Tetsuya. 2020. An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156713
Modern Language Association (MLA)
Chen, Qiliang…[et al.]. An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156713
American Medical Association (AMA)
Chen, Qiliang& Liu, Yaojun& Zhang, Yuanyuan& Jiang, Xinyu& Zhang, Yuqin& Asakawa, Tetsuya. An In Vitro Verification of the Effects of Paeoniflorin on Lipopolysaccharide-Exposed Microglia. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156713
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156713