Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven
المؤلفون المشاركون
Zavala-Sánchez, Miguel A.
Zamilpa, Alejandro
Tortoriello, Jaime
Herrera-Arellano, Armando
Morales, Dulce
Ramirez, Guillermo
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Current antiobesity and antidiabetic tools have been insufficient to curb these diseases and frequently cause side effects; therefore, new pancreatic lipase and α–glucosidase inhibitors could be excellent aids for the prevention and treatment of these diseases.
The aim of this study was to identify, quantify, and characterize the chemical compounds with the highest degree of inhibitory activity of these enzymes, contained in a Ludwigia octovalvis hydroalcoholic extract.
Chemical purification was performed by liquid–liquid separation and column chromatography.
Inhibitory activities were measured in vitro, employing acarbose, orlistat, and a Camellia sinensis hydroalcoholic extract as references.
For structural elucidation, Nuclear Magnetic Resonance was carried out, and High Performance Liquid Chromatography was used to quantify the compounds.
For α–glucosidases, L.
octovalvis hydroalcoholic extract and its ethyl acetate fraction showed half–maximal Inhibitory Concentration (IC50) values of 700 and 250 μg/mL, for lipase, 480 and 718 μg/mL, while C.
sinensis showed 260 and 587 μg/mL.
The most active compounds were identified as ethyl gallate (1, IC50 832 μM) and gallic acid (2, IC50 969 μM); both displayed competitive inhibition of α–glucosidases and isoorientin (3, IC50 201 μM), which displayed uncompetitive inhibition of lipase.
These data could be useful in the development of a novel phytopharmaceutical drug.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Morales, Dulce& Ramirez, Guillermo& Herrera-Arellano, Armando& Tortoriello, Jaime& Zavala-Sánchez, Miguel A.& Zamilpa, Alejandro. 2018. Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156726
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Morales, Dulce…[et al.]. Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1156726
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Morales, Dulce& Ramirez, Guillermo& Herrera-Arellano, Armando& Tortoriello, Jaime& Zavala-Sánchez, Miguel A.& Zamilpa, Alejandro. Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156726
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156726
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر