Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy
المؤلفون المشاركون
Faron, Gilles
Vancutsem, Ellen
Naessens, Anne
Buyl, Ronald
Gucciardo, Leonardo
Foulon, Walter
المصدر
Infectious Diseases in Obstetrics and Gynecology
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-05
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Objective.
This study aimed to compare the qualitative and quantitative reproducibility of quantitative PCR (qPCR) for Ureaplasma species (Ureaplasma spp.) throughout pregnancy and according to the genital sampling site.
Study Design.
Between 5 and 14 weeks of gestation (T1), vaginal, fornix, and two cervical samples were taken.
Sampling was repeated during the 2nd (T2) and 3rd (T3) trimester in randomly selected T1 positive and negative women.
Qualitative and quantitative reproducibility were evaluated using, respectively, Cohen’s kappa (κ) and interclass correlation coefficients (ICC) and repeated measures ANOVA on the log-transformed mean number of DNA copies for each sampling site.
Results.
During T1, 51/127 women were positive for U.
parvum and 8 for U.
urealyticum (4 patients for both).
Sampling was repeated for 44/55 women at T2 and/or T3; 43 (97.7%) remained positive at the three timepoints.
κ ranged between 0.83 and 0.95 and the ICC for cervical samples was 0.86.
Conclusions.
Colonization by Ureaplasma spp.
seems to be very constant during pregnancy and vaginal samples have the highest detection rate.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faron, Gilles& Vancutsem, Ellen& Naessens, Anne& Buyl, Ronald& Gucciardo, Leonardo& Foulon, Walter. 2017. Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy. Infectious Diseases in Obstetrics and Gynecology،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1156835
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faron, Gilles…[et al.]. Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy. Infectious Diseases in Obstetrics and Gynecology No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1156835
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faron, Gilles& Vancutsem, Ellen& Naessens, Anne& Buyl, Ronald& Gucciardo, Leonardo& Foulon, Walter. Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy. Infectious Diseases in Obstetrics and Gynecology. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1156835
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156835
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر