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Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy
Joint Authors
Faron, Gilles
Vancutsem, Ellen
Naessens, Anne
Buyl, Ronald
Gucciardo, Leonardo
Foulon, Walter
Source
Infectious Diseases in Obstetrics and Gynecology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
This study aimed to compare the qualitative and quantitative reproducibility of quantitative PCR (qPCR) for Ureaplasma species (Ureaplasma spp.) throughout pregnancy and according to the genital sampling site.
Study Design.
Between 5 and 14 weeks of gestation (T1), vaginal, fornix, and two cervical samples were taken.
Sampling was repeated during the 2nd (T2) and 3rd (T3) trimester in randomly selected T1 positive and negative women.
Qualitative and quantitative reproducibility were evaluated using, respectively, Cohen’s kappa (κ) and interclass correlation coefficients (ICC) and repeated measures ANOVA on the log-transformed mean number of DNA copies for each sampling site.
Results.
During T1, 51/127 women were positive for U.
parvum and 8 for U.
urealyticum (4 patients for both).
Sampling was repeated for 44/55 women at T2 and/or T3; 43 (97.7%) remained positive at the three timepoints.
κ ranged between 0.83 and 0.95 and the ICC for cervical samples was 0.86.
Conclusions.
Colonization by Ureaplasma spp.
seems to be very constant during pregnancy and vaginal samples have the highest detection rate.
American Psychological Association (APA)
Faron, Gilles& Vancutsem, Ellen& Naessens, Anne& Buyl, Ronald& Gucciardo, Leonardo& Foulon, Walter. 2017. Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy. Infectious Diseases in Obstetrics and Gynecology،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1156835
Modern Language Association (MLA)
Faron, Gilles…[et al.]. Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy. Infectious Diseases in Obstetrics and Gynecology No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1156835
American Medical Association (AMA)
Faron, Gilles& Vancutsem, Ellen& Naessens, Anne& Buyl, Ronald& Gucciardo, Leonardo& Foulon, Walter. Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy. Infectious Diseases in Obstetrics and Gynecology. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1156835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156835