Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes
المؤلفون المشاركون
Li, Huang
Zhang, Yuqin
Yan, Guohong
Sun, Chengtao
Fu, Yanhui
Xu, Wei
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-12-02
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Bauhinia championii (Benth.) Benth.
is a traditional medicinal plant used in China to treat rheumatoid arthritis (RA), especially in She ethnic minority group.
This study focused on the active constituents from the rattan of B.
championii (Benth.) Benth., which possess potential apoptosis effects.
A conventional phytochemical separation method for the isolation of compounds from the ethyl acetate extract of B.
championii was developed.
The procedure involved extraction, liquid–liquid partitioning with ethyl acetate, and subsequent compound purification, respectively.
Additionally, cell viability of dihydrokaempferol found abundantly in it was evaluated in vitro by MTS, and the antiapoptosis effect was evaluated by annexin V/PI staining (Flow Cytometry Analysis) and western blot.
The results showed that nine flavonoids, and five other compounds, were isolated from the ethyl acetate extract of B.
championii and were identified as β-sitosterol (1), 5,6,7,3',4',5'-hexamethoxyflavone (2), 3',4',5,7-tetrahydroxyflavone (3), 5,7,3',4',5'-pentamethoxyflavone (4), 4'-hydroxy-5,7,3',5'-pentamethoxyflavone (5), apigenin (6), liquiritigenin (7), 5, 7-dihydroxylcoumarin (8), 3',4',5,7, -pentamethoxyflavone (9), n-octadecanoate (10), lupine ketone (11), dibutylphthalate (12), dihydrokaempferol (13), and 5,7,3′,5′-tetrahydroxy-6-methylflavanone (14).
Among these compounds, 5-14 were isolated for the first time from B.
championii.
In addition, apoptosis effects of abundant dihydrokaempferol were evaluated in vitro.
Dihydrokaempferol exhibited inhibitory effects on the proliferation of synoviocytes.
Furthermore, dihydrokaempferol promoted Bax and Bad expression, as well as the cleavage of caspase-9, caspase-3, and PARP.
Meanwhile, it inhibited Bcl-2 and Bcl-xL expression.
These findings indicate that dihydrokaempferol isolated from the ethyl acetate extract of B.
championii effectively promotes apoptosis, which is an important process through suppression of apoptotic activity.
The results are encouraging for further studies on the use of B.
championii in the treatment of RA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yuqin& Yan, Guohong& Sun, Chengtao& Li, Huang& Fu, Yanhui& Xu, Wei. 2018. Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156969
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yuqin…[et al.]. Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1156969
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yuqin& Yan, Guohong& Sun, Chengtao& Li, Huang& Fu, Yanhui& Xu, Wei. Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156969
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1156969
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر