Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes
Joint Authors
Li, Huang
Zhang, Yuqin
Yan, Guohong
Sun, Chengtao
Fu, Yanhui
Xu, Wei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Bauhinia championii (Benth.) Benth.
is a traditional medicinal plant used in China to treat rheumatoid arthritis (RA), especially in She ethnic minority group.
This study focused on the active constituents from the rattan of B.
championii (Benth.) Benth., which possess potential apoptosis effects.
A conventional phytochemical separation method for the isolation of compounds from the ethyl acetate extract of B.
championii was developed.
The procedure involved extraction, liquid–liquid partitioning with ethyl acetate, and subsequent compound purification, respectively.
Additionally, cell viability of dihydrokaempferol found abundantly in it was evaluated in vitro by MTS, and the antiapoptosis effect was evaluated by annexin V/PI staining (Flow Cytometry Analysis) and western blot.
The results showed that nine flavonoids, and five other compounds, were isolated from the ethyl acetate extract of B.
championii and were identified as β-sitosterol (1), 5,6,7,3',4',5'-hexamethoxyflavone (2), 3',4',5,7-tetrahydroxyflavone (3), 5,7,3',4',5'-pentamethoxyflavone (4), 4'-hydroxy-5,7,3',5'-pentamethoxyflavone (5), apigenin (6), liquiritigenin (7), 5, 7-dihydroxylcoumarin (8), 3',4',5,7, -pentamethoxyflavone (9), n-octadecanoate (10), lupine ketone (11), dibutylphthalate (12), dihydrokaempferol (13), and 5,7,3′,5′-tetrahydroxy-6-methylflavanone (14).
Among these compounds, 5-14 were isolated for the first time from B.
championii.
In addition, apoptosis effects of abundant dihydrokaempferol were evaluated in vitro.
Dihydrokaempferol exhibited inhibitory effects on the proliferation of synoviocytes.
Furthermore, dihydrokaempferol promoted Bax and Bad expression, as well as the cleavage of caspase-9, caspase-3, and PARP.
Meanwhile, it inhibited Bcl-2 and Bcl-xL expression.
These findings indicate that dihydrokaempferol isolated from the ethyl acetate extract of B.
championii effectively promotes apoptosis, which is an important process through suppression of apoptotic activity.
The results are encouraging for further studies on the use of B.
championii in the treatment of RA.
American Psychological Association (APA)
Zhang, Yuqin& Yan, Guohong& Sun, Chengtao& Li, Huang& Fu, Yanhui& Xu, Wei. 2018. Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156969
Modern Language Association (MLA)
Zhang, Yuqin…[et al.]. Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1156969
American Medical Association (AMA)
Zhang, Yuqin& Yan, Guohong& Sun, Chengtao& Li, Huang& Fu, Yanhui& Xu, Wei. Apoptosis Effects of Dihydrokaempferol Isolated from Bauhinia championii on Synoviocytes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156969
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156969