BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion
المؤلفون المشاركون
Wang, Ling
Feng, Xiaoling
Jiang, Sha
Leung, WingTing
Wang, Ling
Gober, Hans Jürgen
Chen, Lu
Zhang, Yang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-05
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
BuShen HuoXue decoction (BSHXD) has been used to treat patients with unexplained recurrent spontaneous abortion (URSA).
However, the chemical compounds and mechanism by which BSHXD exerts its therapeutic and systemic effects to promote the proliferation of decidual stromal cells (DSCs) has not been elucidated.
This work sought to elucidate the cellular and molecular mechanism of BSHXD in terms of inflammatory factors IL-17A in DSCs in vitro because of the critical roles of inflammation, apoptosis, and immunity in the development and progression of pregnancy loss.
Twelve migratory chemical compounds from BSHXD extract were qualitatively analyzed by high-performance liquid chromatography (HPLC).
DSCs were collected from normal early pregnancy (NEP) and URSA to determine whether BSHXD affects IL-17A/IL17RA via the PI3K/AKT pathway.
Abnormal apoptosis and activated p-AKT were observed in URSA DSCs.
RhIL-17 A, LY294002 (a PI3K pathway inhibitor), and BSHXD were individually or simultaneously administered in NEP DSCs, suggesting that BSHXD restored cell proliferation without excessive stimulation and IL-17A promotes proliferation via the PI3K/AKT pathway.
Using the same intervention in URSA DSCs, qRT-PCR measured the upregulated mRNA levels of IL-17 A/IL-17RA, PI3K, AKT, p-AKT, PTEN, Bcl-2, and Bcl-xL and downregulated mRNA levels of BAD and ACT1 after treatment with BSHXD.
We demonstrated that BSHXD affected IL-17A/IL-17R via PI3K/AKT pathway to promote the proliferative activity of DSCs in URSA.
These results provide a new insight to further clarify the relationship between inflammation and apoptosis and the mechanism of imbalance in the dynamic equilibrium between Th17/Treg immune cells at the maternal-fetal interface.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Feng, Xiaoling& Jiang, Sha& Leung, WingTing& Wang, Ling& Gober, Hans Jürgen& Chen, Lu…[et al.]. 2020. BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157113
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Feng, Xiaoling…[et al.]. BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1157113
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Feng, Xiaoling& Jiang, Sha& Leung, WingTing& Wang, Ling& Gober, Hans Jürgen& Chen, Lu…[et al.]. BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157113
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1157113
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر