BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion
Joint Authors
Wang, Ling
Feng, Xiaoling
Jiang, Sha
Leung, WingTing
Wang, Ling
Gober, Hans Jürgen
Chen, Lu
Zhang, Yang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
BuShen HuoXue decoction (BSHXD) has been used to treat patients with unexplained recurrent spontaneous abortion (URSA).
However, the chemical compounds and mechanism by which BSHXD exerts its therapeutic and systemic effects to promote the proliferation of decidual stromal cells (DSCs) has not been elucidated.
This work sought to elucidate the cellular and molecular mechanism of BSHXD in terms of inflammatory factors IL-17A in DSCs in vitro because of the critical roles of inflammation, apoptosis, and immunity in the development and progression of pregnancy loss.
Twelve migratory chemical compounds from BSHXD extract were qualitatively analyzed by high-performance liquid chromatography (HPLC).
DSCs were collected from normal early pregnancy (NEP) and URSA to determine whether BSHXD affects IL-17A/IL17RA via the PI3K/AKT pathway.
Abnormal apoptosis and activated p-AKT were observed in URSA DSCs.
RhIL-17 A, LY294002 (a PI3K pathway inhibitor), and BSHXD were individually or simultaneously administered in NEP DSCs, suggesting that BSHXD restored cell proliferation without excessive stimulation and IL-17A promotes proliferation via the PI3K/AKT pathway.
Using the same intervention in URSA DSCs, qRT-PCR measured the upregulated mRNA levels of IL-17 A/IL-17RA, PI3K, AKT, p-AKT, PTEN, Bcl-2, and Bcl-xL and downregulated mRNA levels of BAD and ACT1 after treatment with BSHXD.
We demonstrated that BSHXD affected IL-17A/IL-17R via PI3K/AKT pathway to promote the proliferative activity of DSCs in URSA.
These results provide a new insight to further clarify the relationship between inflammation and apoptosis and the mechanism of imbalance in the dynamic equilibrium between Th17/Treg immune cells at the maternal-fetal interface.
American Psychological Association (APA)
Feng, Xiaoling& Jiang, Sha& Leung, WingTing& Wang, Ling& Gober, Hans Jürgen& Chen, Lu…[et al.]. 2020. BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157113
Modern Language Association (MLA)
Feng, Xiaoling…[et al.]. BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1157113
American Medical Association (AMA)
Feng, Xiaoling& Jiang, Sha& Leung, WingTing& Wang, Ling& Gober, Hans Jürgen& Chen, Lu…[et al.]. BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the PI3KAKT Pathway in Unexplained Recurrent Spontaneous Abortion. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157113
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157113