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Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection
المؤلفون المشاركون
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-13
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties.
They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives.
The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis.
Method validation controls were performed and recovery experiments were assayed.
The data was statically treated and compared with published works.
The current method allowed a feasible identification of indole glucosinolates and it was possible to identify accurately three indole glucosinolate compounds (glucobrassicin, 4-methoxyglucobrassicin, and 1-methoxyglucobrassicin) in roots of Chinese cabbage.
The method demonstrated a good linearity (R2>0.99), a good precision, and selectivity sensitivity.
In conclusion, this protocol provides an accessible method to extract and quantify glucosinolates in plant samples.
Thus, based on our results, the method is valid and can be extended to other plant or food matrices.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Aires, Alfredo& Carvalho, Rosa. 2017. Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1157573
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Aires, Alfredo& Carvalho, Rosa. Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1157573
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Aires, Alfredo& Carvalho, Rosa. Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1157573
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1157573
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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