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Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection
Joint Authors
Source
International Journal of Analytical Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties.
They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives.
The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis.
Method validation controls were performed and recovery experiments were assayed.
The data was statically treated and compared with published works.
The current method allowed a feasible identification of indole glucosinolates and it was possible to identify accurately three indole glucosinolate compounds (glucobrassicin, 4-methoxyglucobrassicin, and 1-methoxyglucobrassicin) in roots of Chinese cabbage.
The method demonstrated a good linearity (R2>0.99), a good precision, and selectivity sensitivity.
In conclusion, this protocol provides an accessible method to extract and quantify glucosinolates in plant samples.
Thus, based on our results, the method is valid and can be extended to other plant or food matrices.
American Psychological Association (APA)
Aires, Alfredo& Carvalho, Rosa. 2017. Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1157573
Modern Language Association (MLA)
Aires, Alfredo& Carvalho, Rosa. Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1157573
American Medical Association (AMA)
Aires, Alfredo& Carvalho, Rosa. Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapa Subsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1157573
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157573