Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation
المؤلفون المشاركون
Wang, Jian’an
Huang, Lufen
Ren, Qiang
Wang, Yanjun
Zhou, Lirun
Fu, Yingjie
Sai, Chunmei
Pella, Shafii Shaibu
Guo, Yingying
Gao, Li-Na
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-15
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The roots of Scrophularia ningpoensis Hemsl.
are a famous traditional Chinese medicinal herb and are also used as health food.
However, information about polysaccharides from S.
ningpoensis (SNPS) is very limited.
We applied the ultrasonic-assisted extraction (UAE) process to extract SNPS.
The UAE conditions were optimized using single-factor experiments and response surface analysis.
Under the optimized conditions of ultrasonic power of 550 W, extraction time of 26 min, and extraction temperature at 50°C, the highest yield of 13.47% ± 1.63% was obtained, which was in accordance with the predicted value of 13.71%.
In comparison with traditional hot water extraction, the optimized UAE method significantly increased the extraction yield with lower extraction temperature and shorter extraction time.
Furthermore, the in vitro antioxidant evaluation showed that EC50 values of SNPS were 2.43 ± 0.21, 4.40 ± 0.35, and 0.56 ± 0.062 mg/mL for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) radical, hydroxyl free radical, and 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, respectively.
The anti-inflammatory potential of SNPS was detected in lipopolysaccharide (LPS) induced ICR mice.
Real-time reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay showed that SNPS significantly improved LPS-stimulated inflammatory response by decreasing mRNA and protein expression of interleukin (IL)-6 and tumour necrosis factor (TNF)-α in a dose-dependent manner.
In conclusion, the extraction process of SNPS established in this study is reliable, and SNPS possesses potential antioxidant and anti-inflammatory activities, which will provide a theoretical basis for guiding the clinical application of S.
ningpoensis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Jian’an& Huang, Lufen& Ren, Qiang& Wang, Yanjun& Zhou, Lirun& Fu, Yingjie…[et al.]. 2020. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158256
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Jian’an…[et al.]. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1158256
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Jian’an& Huang, Lufen& Ren, Qiang& Wang, Yanjun& Zhou, Lirun& Fu, Yingjie…[et al.]. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158256
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158256
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر