![](/images/graphics-bg.png)
Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation
Joint Authors
Wang, Jian’an
Huang, Lufen
Ren, Qiang
Wang, Yanjun
Zhou, Lirun
Fu, Yingjie
Sai, Chunmei
Pella, Shafii Shaibu
Guo, Yingying
Gao, Li-Na
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The roots of Scrophularia ningpoensis Hemsl.
are a famous traditional Chinese medicinal herb and are also used as health food.
However, information about polysaccharides from S.
ningpoensis (SNPS) is very limited.
We applied the ultrasonic-assisted extraction (UAE) process to extract SNPS.
The UAE conditions were optimized using single-factor experiments and response surface analysis.
Under the optimized conditions of ultrasonic power of 550 W, extraction time of 26 min, and extraction temperature at 50°C, the highest yield of 13.47% ± 1.63% was obtained, which was in accordance with the predicted value of 13.71%.
In comparison with traditional hot water extraction, the optimized UAE method significantly increased the extraction yield with lower extraction temperature and shorter extraction time.
Furthermore, the in vitro antioxidant evaluation showed that EC50 values of SNPS were 2.43 ± 0.21, 4.40 ± 0.35, and 0.56 ± 0.062 mg/mL for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) radical, hydroxyl free radical, and 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, respectively.
The anti-inflammatory potential of SNPS was detected in lipopolysaccharide (LPS) induced ICR mice.
Real-time reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay showed that SNPS significantly improved LPS-stimulated inflammatory response by decreasing mRNA and protein expression of interleukin (IL)-6 and tumour necrosis factor (TNF)-α in a dose-dependent manner.
In conclusion, the extraction process of SNPS established in this study is reliable, and SNPS possesses potential antioxidant and anti-inflammatory activities, which will provide a theoretical basis for guiding the clinical application of S.
ningpoensis.
American Psychological Association (APA)
Wang, Jian’an& Huang, Lufen& Ren, Qiang& Wang, Yanjun& Zhou, Lirun& Fu, Yingjie…[et al.]. 2020. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158256
Modern Language Association (MLA)
Wang, Jian’an…[et al.]. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1158256
American Medical Association (AMA)
Wang, Jian’an& Huang, Lufen& Ren, Qiang& Wang, Yanjun& Zhou, Lirun& Fu, Yingjie…[et al.]. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, Antioxidant, and Anti-Inflammatory Evaluation. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158256