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Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand
المؤلفون المشاركون
Poonchareon, Kritchai
Pulsrikarn, Chaiwat
Nuanmuang, Narong
Khamai, Phichaya
المصدر
International Journal of Microbiology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-17
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Salmonella enterica Serotype 4,[5],12:i:-, a monophasic variant of S.
Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals.
BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)5-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification condition), in differentiating genetic relatedness among S.
4,[5],12:i:- isolates from feces of hospitalized patients (n=12) and isolates from minced pork samples of S.
4,[5],12:i:- (n=6), S.
Typhimurium (n=6), and Salmonella Serogroup B (n=4) collected from different regions of northern Thailand.
Construction of phylogenetic trees from amplicon size patterns allowed allocation of Salmonella isolates into clusters of similar genetic relatedness, with BOX-PCR generating more unique clusters for each serotype than the other three typing methods.
BOX-, (GTG)5-, and REP-PCR indicated significant genetic relatedness between S.
4,[5],12:i:- isolates 1 and 9 from hospitalized patients and S.
4,[5],12:i:- isolate en 29 from minced pork, suggesting a possible route of transmission.
Thus, BOX-PCR provides a suitable molecular typing method for discriminating genetic relatedness among Salmonella spp.
of the same and different serotypes and should be suitable for application in typing and tracking route of transmission in Salmonella outbreaks.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Poonchareon, Kritchai& Pulsrikarn, Chaiwat& Nuanmuang, Narong& Khamai, Phichaya. 2019. Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand. International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1166226
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Poonchareon, Kritchai…[et al.]. Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand. International Journal of Microbiology No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1166226
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Poonchareon, Kritchai& Pulsrikarn, Chaiwat& Nuanmuang, Narong& Khamai, Phichaya. Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand. International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1166226
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166226
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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