Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand
Joint Authors
Poonchareon, Kritchai
Pulsrikarn, Chaiwat
Nuanmuang, Narong
Khamai, Phichaya
Source
International Journal of Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Salmonella enterica Serotype 4,[5],12:i:-, a monophasic variant of S.
Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals.
BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)5-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification condition), in differentiating genetic relatedness among S.
4,[5],12:i:- isolates from feces of hospitalized patients (n=12) and isolates from minced pork samples of S.
4,[5],12:i:- (n=6), S.
Typhimurium (n=6), and Salmonella Serogroup B (n=4) collected from different regions of northern Thailand.
Construction of phylogenetic trees from amplicon size patterns allowed allocation of Salmonella isolates into clusters of similar genetic relatedness, with BOX-PCR generating more unique clusters for each serotype than the other three typing methods.
BOX-, (GTG)5-, and REP-PCR indicated significant genetic relatedness between S.
4,[5],12:i:- isolates 1 and 9 from hospitalized patients and S.
4,[5],12:i:- isolate en 29 from minced pork, suggesting a possible route of transmission.
Thus, BOX-PCR provides a suitable molecular typing method for discriminating genetic relatedness among Salmonella spp.
of the same and different serotypes and should be suitable for application in typing and tracking route of transmission in Salmonella outbreaks.
American Psychological Association (APA)
Poonchareon, Kritchai& Pulsrikarn, Chaiwat& Nuanmuang, Narong& Khamai, Phichaya. 2019. Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand. International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1166226
Modern Language Association (MLA)
Poonchareon, Kritchai…[et al.]. Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand. International Journal of Microbiology No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1166226
American Medical Association (AMA)
Poonchareon, Kritchai& Pulsrikarn, Chaiwat& Nuanmuang, Narong& Khamai, Phichaya. Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand. International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1166226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166226