Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
المؤلفون المشاركون
Tong, Chunyi
Liu, Qingxin
Fan, Jialong
Zhou, Chuang
Wang, Liqun
Zhao, Bin
Zhang, Haibin
Liu, Bin
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-02
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer.
With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM.
Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues.
The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy.
In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference.
Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method.
The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Qingxin& Fan, Jialong& Zhou, Chuang& Wang, Liqun& Zhao, Bin& Zhang, Haibin…[et al.]. 2018. Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1166362
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Qingxin…[et al.]. Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1166362
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Qingxin& Fan, Jialong& Zhou, Chuang& Wang, Liqun& Zhao, Bin& Zhang, Haibin…[et al.]. Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1166362
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166362
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر