Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS)
المؤلفون المشاركون
Khelfi, Abderrezak
Azzouz, Mohammed
Abtroun, Rania
Reggabi, Mohammed
Alamir, Berkahoum
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-02
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background and Objective.
In this study, turbo-ion spray as an interface of tandem mass spectrometry (MS/MS) was performed for sensitive and accurate quantification of chlorpromazine, haloperidol, levomepromazine, olanzapine, risperidone, and sulpiride in plasma samples.
Methods.
Separation was performed by gradient reversed phase high-performance liquid chromatography using a mobile phase containing ammonium formiate 2 mM, pH 2.7, and acetonitrile flowing through a Restek PFP Propyl C18 analytical column (50 mm×2.1 mm i.d.) with particle size of 5 µm, at a flow rate of 800 µL/min.
Positive ion fragments were detected in multiple reaction monitoring (MRM) mode.
Sample preparation was achieved by solid phase extraction (SPE) (Oasis HLB).
Results.
Mean extraction recoveries ranged from 82.75% to 100.96%.
The standard calibration curves showed an excellent linearity, covering subtherapeutic, therapeutic, and toxic ranges.
Intraday and interday validation using quality control (QC) samples were performed.
The inaccuracy and imprecision were below 12% at all concentration levels.
The limits of detection (LOD) and quantification (LOQ) for all analytes were under therapeutic ranges for all tested analytes.
Thus, the proposed method was sensitive enough for the detection and determination of subtherapeutic levels of these antipsychotics in plasma samples.
No interference of endogenous or exogenous molecules was observed and no carryover effects were recorded.
Conclusion.
According to the results, the proposed method is simple, specific, linear, accurate, and precise and can be applied for antipsychotic analysis in clinical routine.
This method was applied for the determination of the tested antipsychotics in plasma samples taken from 71 individuals.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khelfi, Abderrezak& Azzouz, Mohammed& Abtroun, Rania& Reggabi, Mohammed& Alamir, Berkahoum. 2018. Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS). International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1166441
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khelfi, Abderrezak…[et al.]. Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS). International Journal of Analytical Chemistry No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1166441
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khelfi, Abderrezak& Azzouz, Mohammed& Abtroun, Rania& Reggabi, Mohammed& Alamir, Berkahoum. Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS). International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1166441
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166441
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر