Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS)
Joint Authors
Khelfi, Abderrezak
Azzouz, Mohammed
Abtroun, Rania
Reggabi, Mohammed
Alamir, Berkahoum
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background and Objective.
In this study, turbo-ion spray as an interface of tandem mass spectrometry (MS/MS) was performed for sensitive and accurate quantification of chlorpromazine, haloperidol, levomepromazine, olanzapine, risperidone, and sulpiride in plasma samples.
Methods.
Separation was performed by gradient reversed phase high-performance liquid chromatography using a mobile phase containing ammonium formiate 2 mM, pH 2.7, and acetonitrile flowing through a Restek PFP Propyl C18 analytical column (50 mm×2.1 mm i.d.) with particle size of 5 µm, at a flow rate of 800 µL/min.
Positive ion fragments were detected in multiple reaction monitoring (MRM) mode.
Sample preparation was achieved by solid phase extraction (SPE) (Oasis HLB).
Results.
Mean extraction recoveries ranged from 82.75% to 100.96%.
The standard calibration curves showed an excellent linearity, covering subtherapeutic, therapeutic, and toxic ranges.
Intraday and interday validation using quality control (QC) samples were performed.
The inaccuracy and imprecision were below 12% at all concentration levels.
The limits of detection (LOD) and quantification (LOQ) for all analytes were under therapeutic ranges for all tested analytes.
Thus, the proposed method was sensitive enough for the detection and determination of subtherapeutic levels of these antipsychotics in plasma samples.
No interference of endogenous or exogenous molecules was observed and no carryover effects were recorded.
Conclusion.
According to the results, the proposed method is simple, specific, linear, accurate, and precise and can be applied for antipsychotic analysis in clinical routine.
This method was applied for the determination of the tested antipsychotics in plasma samples taken from 71 individuals.
American Psychological Association (APA)
Khelfi, Abderrezak& Azzouz, Mohammed& Abtroun, Rania& Reggabi, Mohammed& Alamir, Berkahoum. 2018. Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS). International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1166441
Modern Language Association (MLA)
Khelfi, Abderrezak…[et al.]. Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS). International Journal of Analytical Chemistry No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1166441
American Medical Association (AMA)
Khelfi, Abderrezak& Azzouz, Mohammed& Abtroun, Rania& Reggabi, Mohammed& Alamir, Berkahoum. Determination of Chlorpromazine, Haloperidol, Levomepromazine, Olanzapine, Risperidone, and Sulpiride in Human Plasma by Liquid ChromatographyTandem Mass Spectrometry (LC-MSMS). International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1166441
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166441