Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway
المؤلفون المشاركون
Xu, Xin
Li, Guoliang
Zhu, Quanli
Ma, Junjian
Pan, Zhijian
Wu, Wei
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-10
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Somatostatin (SST) has a protective role in intestinal injury, inflammatory response, and intestinal mucosal barrier in rats with acute pancreatitis.
However, its function in sepsis-induced intestinal barrier dysfunction remains largely unknown.
A mouse sepsis model was constructed, and SST was injected into the tail vein.
Then, hematoxylin and eosin staining (HE) was used to detect the intestinal barrier dysfunction.
Enzyme-linked immunosorbent assay was used to detect the level of tumor necrosis factor α- (TNF-) α, interleukin- (IL-) 6, and interleukin- (IL-) 10 in the ileum.
Expressions of tight junction proteins, zonula occludens- (ZO-) 1 and Claudin-1, and NF-κB p65 in the ileum were detected using western blot and immunohistochemistry as needed.
Furthermore, JSH-23 as an inhibitor of the NF-κB pathway was injected into sepsis mice with SST or not.
Mice with sepsis showed an obvious intestinal barrier dysfunction with decreasing specific somatostatin receptor subtype (SSTRs), and increasing TNF-α, IL-6, and IL-10 in the ileum.
SST could relieve the injury, the decrease of SSTRs, and the increase of TNF-α and IL-6 induced by sepsis and also further enhanced the expression of IL-10.
Further analysis showed that ZO-1 and Claudin-1 were reduced in the ileum by sepsis but enhanced by SST.
NF-κB p65 was promoted in the ileum by sepsis but inhibited by SST.
Further experiments confirmed that NF-κB inhibitor JSH-23 could repair the intestinal barrier dysfunction and enhance the protective effect of SST on the intestinal barrier.
SST, with a protective effect on intestinal barrier dysfunction through suppression of NF-κB, could be a potential therapeutic drug for sepsis-induced intestinal barrier dysfunction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Xin& Zhu, Quanli& Li, Guoliang& Ma, Junjian& Pan, Zhijian& Wu, Wei. 2020. Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1166722
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Xin…[et al.]. Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway. Gastroenterology Research and Practice No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1166722
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Xin& Zhu, Quanli& Li, Guoliang& Ma, Junjian& Pan, Zhijian& Wu, Wei. Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1166722
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166722
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر