miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4
المؤلفون المشاركون
Tian, Shuxia
Chen, Min
Wang, Bing
Han, Yonglong
Shang, Haonan
Chen, Junming
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Aims.
Fibroblast growth factor receptor 4 (FGFR4) is a key mediator that protects the liver from chronic injury.
MicroRNA-7 (miR-7) is a tumor suppressor and associated with lipid homeostasis in the liver.
This study was designed to examine the role of the miR-7-5p/FGFR4 axis in liver fibrogenesis.
Methods.
TargetScan was employed to predict microRNAs that targeted FGFR4 on the 3′-untranslated region (3′-UTR).
miR-7-5p and FGFR4 expression in pathological liver tissues and LX-2 cells was determined using qRT-PCR and an immunoblotting assay.
A dual-luciferase assay was conducted to validate the target prediction.
A Cell Counting Lit-8 assay was performed to assess the proliferation ability of LX-2 cells.
Hydroxyproline content in LX-2 cells was measured using a hydroxyproline assay.
The expression of hepatic stellate cell (HSC) activation markers was examined using qRT-PCR and an immunoblotting assay.
Results.
FGFR4 was a putative target of miR-7-5p.
In LX-2 cells, miR-7-5p targeted FGFR4 by binding to 3′-UTR.
FGFR4 was downregulated, but miR-7-5p was markedly enhanced in the liver samples as the degree of liver fibrosis rose.
miR-7-5p was negatively associated with FGFR4 expression in liver tissues.
The miR-7-5p inhibitor blocked the lipopolysaccharide-induced proliferation and activation of LX-2 cells, and FGFR4 overexpression inhibited LX-2 cell proliferation and activation triggered by miR-7-5p.
Conclusion.
miR-7-5p promotes HSC proliferation and activation by downregulating FGFR4.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tian, Shuxia& Chen, Min& Wang, Bing& Han, Yonglong& Shang, Haonan& Chen, Junming. 2020. miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166877
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tian, Shuxia…[et al.]. miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4. Gastroenterology Research and Practice No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1166877
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tian, Shuxia& Chen, Min& Wang, Bing& Han, Yonglong& Shang, Haonan& Chen, Junming. miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166877
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166877
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر