miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4
Joint Authors
Tian, Shuxia
Chen, Min
Wang, Bing
Han, Yonglong
Shang, Haonan
Chen, Junming
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aims.
Fibroblast growth factor receptor 4 (FGFR4) is a key mediator that protects the liver from chronic injury.
MicroRNA-7 (miR-7) is a tumor suppressor and associated with lipid homeostasis in the liver.
This study was designed to examine the role of the miR-7-5p/FGFR4 axis in liver fibrogenesis.
Methods.
TargetScan was employed to predict microRNAs that targeted FGFR4 on the 3′-untranslated region (3′-UTR).
miR-7-5p and FGFR4 expression in pathological liver tissues and LX-2 cells was determined using qRT-PCR and an immunoblotting assay.
A dual-luciferase assay was conducted to validate the target prediction.
A Cell Counting Lit-8 assay was performed to assess the proliferation ability of LX-2 cells.
Hydroxyproline content in LX-2 cells was measured using a hydroxyproline assay.
The expression of hepatic stellate cell (HSC) activation markers was examined using qRT-PCR and an immunoblotting assay.
Results.
FGFR4 was a putative target of miR-7-5p.
In LX-2 cells, miR-7-5p targeted FGFR4 by binding to 3′-UTR.
FGFR4 was downregulated, but miR-7-5p was markedly enhanced in the liver samples as the degree of liver fibrosis rose.
miR-7-5p was negatively associated with FGFR4 expression in liver tissues.
The miR-7-5p inhibitor blocked the lipopolysaccharide-induced proliferation and activation of LX-2 cells, and FGFR4 overexpression inhibited LX-2 cell proliferation and activation triggered by miR-7-5p.
Conclusion.
miR-7-5p promotes HSC proliferation and activation by downregulating FGFR4.
American Psychological Association (APA)
Tian, Shuxia& Chen, Min& Wang, Bing& Han, Yonglong& Shang, Haonan& Chen, Junming. 2020. miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166877
Modern Language Association (MLA)
Tian, Shuxia…[et al.]. miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4. Gastroenterology Research and Practice No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1166877
American Medical Association (AMA)
Tian, Shuxia& Chen, Min& Wang, Bing& Han, Yonglong& Shang, Haonan& Chen, Junming. miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166877