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Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
المؤلفون المشاركون
Zhang, Dianliang
Wang, Hanlin
Li, Chang
Jiang, Yingjian
Li, Hongbo
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Aim.
To reveal the role of bacterial translocation (BT) and autophagy in severe acute pancreatitis-induced acute lung injury (SAP-ALI).
Methods.
Rats were separated into a control (sham-operation) group (n=10) and a SAP group (n=30).
Sodium taurocholate (5%) was retrogradely injected into the cholangiopancreatic duct to induce SAP-ALI in rats.
Then, 16S rDNA sequencing was used to detect bacterial translocation (BT).
Hematoxylin eosin staining (HE) was used to detect morphological changes to the pancreas, intestine, and lung.
And lung tissue wet/dry weight ratio (W/D ratio) was used to assess the extent of pulmonary edema.
The expressions of LC3II and Beclin1 proteins were analyzed by western blot and immunofluorescence.
Glutathione peroxidase (GPx), malondialdehyde (MDA), and superoxide dismutase (SOD) were used to assess oxidative stress in lung tissue.
Results.
Levels of TNF-α, IL-6, lipase, and amylase in the SAP group were significantly higher than those in the control group (P<0.01).
Histopathological score and W/D ratio of the lung in the SAP-BT(+) group were significantly higher than that in the SAP-BT(-) group (P<0.01).
LC3II expression was higher in the SAP-BT(-) group than that in the SAP-BT(+) group (P<0.01).
The results were consistent with those of LC3II immunofluorescence assay.
The expression of Beclin1 was similar to that of LC3II (P<0.01).
MDA content in the SAP-BT(+) group was significantly higher than that in the SAP-BT(-) group (P<0.01), whereas SOD and GPX activities were opposite (P<0.01).
Conclusions.
BT can aggravate SAP-ALI with the increasing oxidative stress level, which may be related to the decrease of autophagy level.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Hanlin& Li, Chang& Jiang, Yingjian& Li, Hongbo& Zhang, Dianliang. 2020. Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1167099
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Hanlin…[et al.]. Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1167099
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Hanlin& Li, Chang& Jiang, Yingjian& Li, Hongbo& Zhang, Dianliang. Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1167099
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167099
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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