Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis
Joint Authors
Zhang, Dianliang
Wang, Hanlin
Li, Chang
Jiang, Yingjian
Li, Hongbo
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
To reveal the role of bacterial translocation (BT) and autophagy in severe acute pancreatitis-induced acute lung injury (SAP-ALI).
Methods.
Rats were separated into a control (sham-operation) group (n=10) and a SAP group (n=30).
Sodium taurocholate (5%) was retrogradely injected into the cholangiopancreatic duct to induce SAP-ALI in rats.
Then, 16S rDNA sequencing was used to detect bacterial translocation (BT).
Hematoxylin eosin staining (HE) was used to detect morphological changes to the pancreas, intestine, and lung.
And lung tissue wet/dry weight ratio (W/D ratio) was used to assess the extent of pulmonary edema.
The expressions of LC3II and Beclin1 proteins were analyzed by western blot and immunofluorescence.
Glutathione peroxidase (GPx), malondialdehyde (MDA), and superoxide dismutase (SOD) were used to assess oxidative stress in lung tissue.
Results.
Levels of TNF-α, IL-6, lipase, and amylase in the SAP group were significantly higher than those in the control group (P<0.01).
Histopathological score and W/D ratio of the lung in the SAP-BT(+) group were significantly higher than that in the SAP-BT(-) group (P<0.01).
LC3II expression was higher in the SAP-BT(-) group than that in the SAP-BT(+) group (P<0.01).
The results were consistent with those of LC3II immunofluorescence assay.
The expression of Beclin1 was similar to that of LC3II (P<0.01).
MDA content in the SAP-BT(+) group was significantly higher than that in the SAP-BT(-) group (P<0.01), whereas SOD and GPX activities were opposite (P<0.01).
Conclusions.
BT can aggravate SAP-ALI with the increasing oxidative stress level, which may be related to the decrease of autophagy level.
American Psychological Association (APA)
Wang, Hanlin& Li, Chang& Jiang, Yingjian& Li, Hongbo& Zhang, Dianliang. 2020. Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1167099
Modern Language Association (MLA)
Wang, Hanlin…[et al.]. Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1167099
American Medical Association (AMA)
Wang, Hanlin& Li, Chang& Jiang, Yingjian& Li, Hongbo& Zhang, Dianliang. Effects of Bacterial Translocation and Autophagy on Acute Lung Injury Induced by Severe Acute Pancreatitis. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1167099
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167099