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Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation
المؤلفون المشاركون
Guénard, Frédéric
Vohl, Marie-Claude
Côté, Julie Anne
Lessard, Julie
Lapointe, Marc
Biron, Simon
Tchernof, André
المصدر
International Journal of Genomics
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-19
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objective.
To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture.
Methods.
Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI.
Isolated adipocytes were dedifferentiated in ceiling culture.
Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays.
Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions.
Results.
Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12.
Most of these genes coded for proteins involved in adipocyte functions (LIPE, PLIN1, DGAT2, PNPLA2, ADIPOQ, CEBPA, LPL, FABP4, SCD, INSR, and LEP).
Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (WNT5A, KITLG, and FGF5).
Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1).
Conclusion.
Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Côté, Julie Anne& Guénard, Frédéric& Lessard, Julie& Lapointe, Marc& Biron, Simon& Vohl, Marie-Claude…[et al.]. 2017. Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1167101
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Côté, Julie Anne…[et al.]. Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation. International Journal of Genomics No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1167101
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Côté, Julie Anne& Guénard, Frédéric& Lessard, Julie& Lapointe, Marc& Biron, Simon& Vohl, Marie-Claude…[et al.]. Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1167101
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167101
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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