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Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation
Joint Authors
Guénard, Frédéric
Vohl, Marie-Claude
Côté, Julie Anne
Lessard, Julie
Lapointe, Marc
Biron, Simon
Tchernof, André
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture.
Methods.
Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI.
Isolated adipocytes were dedifferentiated in ceiling culture.
Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays.
Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions.
Results.
Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12.
Most of these genes coded for proteins involved in adipocyte functions (LIPE, PLIN1, DGAT2, PNPLA2, ADIPOQ, CEBPA, LPL, FABP4, SCD, INSR, and LEP).
Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (WNT5A, KITLG, and FGF5).
Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1).
Conclusion.
Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle.
American Psychological Association (APA)
Côté, Julie Anne& Guénard, Frédéric& Lessard, Julie& Lapointe, Marc& Biron, Simon& Vohl, Marie-Claude…[et al.]. 2017. Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1167101
Modern Language Association (MLA)
Côté, Julie Anne…[et al.]. Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation. International Journal of Genomics No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1167101
American Medical Association (AMA)
Côté, Julie Anne& Guénard, Frédéric& Lessard, Julie& Lapointe, Marc& Biron, Simon& Vohl, Marie-Claude…[et al.]. Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1167101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167101