Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells

المؤلفون المشاركون

Nielsen, Ditte Søvsø Gundelund
Fredborg, Marlene
Andersen, Vibeke
Purup, Stig

المصدر

International Journal of Inflammation

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-10-26

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

Inflammatory bowel diseases (IBD) are chronic inflammatory diseases involving all or part of the gastrointestinal tract.

The stress-activated serine-threonine protein kinase D1 (PKD1) protein has previously been implicated in intestinal immune regulation.

The objective of this study was to evaluate the effects of human PKD1 in relation to intestinal inflammation, using a co-culture model of intestinal epithelial Caco-2 cells and RAW264.7 macrophages.

An inflammatory response was induced in the macrophages by lipopolysaccharide (LPS), upregulating the expression of tumour necrosis factor alpha (TNF-α), interleukin- (IL-) 1β, and IL-6 besides increasing the secretion of TNF-α protein.

The effect of administering PKD1 to Caco-2 was evaluated in relation to both amelioration of inflammation and the ability to suppress inflammation initiation.

Administration of PKD1 (10–100 ng/ml) following induction of inflammation induced downregulation of TNF-α expression in RAW264.7 cells.

In addition, PKD1 administered for 3 h prior to LPS stimulation reduced the subsequent inflammatory response through downregulation of TNF-α, IL-1β, and IL-6 in RAW264.7 cells.

These results demonstrate a potential role of PKD1 in the intercellular communication between intestinal epithelial and immune cells, proposing a protective effect of PKD1 on the induction of an inflammatory response in macrophages, an important aspect during the pathogenesis of IBD.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Nielsen, Ditte Søvsø Gundelund& Fredborg, Marlene& Andersen, Vibeke& Purup, Stig. 2017. Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells. International Journal of Inflammation،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1167507

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Nielsen, Ditte Søvsø Gundelund…[et al.]. Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells. International Journal of Inflammation No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1167507

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Nielsen, Ditte Søvsø Gundelund& Fredborg, Marlene& Andersen, Vibeke& Purup, Stig. Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells. International Journal of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1167507

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1167507